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pbabed puromycin vectors  (TaKaRa)


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    TaKaRa pbabed puromycin vectors
    Pbabed Puromycin Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabed+puromycin+vectors/Puromycin/bio_rxiv__2025__06__25__661481-56-2-18
    Average 96 stars, based on 3506 article reviews
    pbabed puromycin vectors - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Mutations within the predicted fragment-binding region of FAM83G/SACK1G abolish its interaction with the Ser/Thr kinase CK1α
    Article Snippet: Briefly, the pBabeD-puromycin vectors encoding the specific mutants (6 μg), pCMV5-gag/pol (3.2 μg) and pCMV5-VSV-G plasmids (2.8 μg) (Clontech) were co-transfected into ∼60-70% confluent HEK-293FT cells in a 10-cm diameter dish using PEI reagent as previously described – .



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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, <t>TERT-HA,</t> <t>DNTERT,</t> and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .
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    Image Search Results


    ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, TERT-HA, DNTERT, and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .

    Journal: Frontiers in Aging

    Article Title: Membrane transporter progressive ankylosis protein homologue ( ANKH / Ank ) partially mediates senescence-derived extracellular citrate and is regulated by DNA damage, inflammation, and ageing

    doi: 10.3389/fragi.2025.1583288

    Figure Lengend Snippet: ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, TERT-HA, DNTERT, and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .

    Article Snippet: pBABE retroviral vectors on the puromycin-resistant backbone expressing TERT and TERT-HA , the dominant-negative catalytically dead TERT DNTERT , or the empty vector were obtained from Addgene Europe (Teddington, Middlesex, UK; Cat# 1771, 1772 and 1775, respectively).

    Techniques: Expressing, Marker, Standard Deviation, Staining